The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. UV흡광 결과보다 민감도 우수. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. (2012). Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. AccuBlue® RNA Quantitation Kit Details. 3. Bioz Stars score: 86/100, based on 1 PubMed citations. quant it picogreen dsdna assay kit reagents. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. 参考資料. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. 3,201 3. Bioz Stars score: 86/100, based on 1 PubMed citations. No. Catalog Number. PicoGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Qubit® dsDNA HS Assay Kit. Bioz Stars score: 86/100, based on 1 PubMed citations. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. 995 mL TE for the . 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. Natural cures with herbs are. Skip to main list 美谷分子仪器（上海）有限公司. Thermo Fisher quant it picogreen dsdna assay kit. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. 2 to 100 ng) Assay Kit were used with a Qubit 3. 0 fluorometer and Quant-iT PicoGreen dsDNA. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Qubit™ dsDNA Quantification Assay Kits. Three kits covering a wide range of dsDNA concentrations. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 製品番号（カタログ番号）： P7581. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. Catalog Number. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Figure 2. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. Fluorescence was measured at. For duplicate-well droplet. , QuantIt PicoGreen dsDNA assay). Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quantify nucleic acids and proteins using four user-selectable fluorescent. 99. The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). Figure 1. Thermo Fisher quant it picogreen dsdna quantification reagent kit Quant It Picogreen Dsdna Quantification Reagent Kit, supplied by Thermo Fisher, used in various techniques. You will need 100 μl diluted PicoGreen reagent per well. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:DNA concentration was quantified with Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) on a Tecan plate reader. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. 005% Triton X-100, Tween-20, or Brij. 99 to $1999. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. ru by: 1. Pico488 allows to quantitate as little as low pg/mL of dsDNA. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). PicoGreen Solution must be diluted fresh before each assay run. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. • Suitable for use in 96- and 384-well plate formats. Rapidly detect as little as 0. After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). Rapidly detect as little as 0. 製品概要. 10. Begin typing to search, use arrow keys to navigate, Enter to select. Catalog Number. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. The fluorescence intensity of PicoGreen was measured to determine the. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. P11495. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Description. Rapidly detect as little as 0. Description. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. PicoGreen dsDNA quantitation reagents are. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. BR reagent (Component A) 1. Using this data as a starting point, the optimal conditions (e. The linear detection range of the PicoGreen® assay in the 20/20n extends over three orders of magnitude in DNA concentration - from 450 pg/mLFor DNA quantification with PicoGreen (PG), Quant-iT™ PicoGreen® dsDNA Reagents and Kits (Invitrogen – Molecular Probes, Eugene, OR, USA) were used. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. Rapidly detect as little as 0. 99 to $1999. Both fluorescent assays were performed with a Qubit fluorometer. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. io |. DNA was quantified by the Quant-it PicoGreen dsDNA Assay Kit (Invitrogen Life technologies, UK) according to the manufacturer’s protocol. Katalognummer: P11495. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Unit Size. 0. Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. • Specific for dsDNA in the presence of RNA. Quantitation using Quant-iT Assay Kits. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. n = 3. Product Type. • Specific for dsDNA in the presence of RNA. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Lambda DNA was used to generate the. low-range. The assay is linear over three orders of magnitude and has little sequence dependence. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. Catalog Number. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. 12. Quant-iT PicoGreen dsDNA Assay Kit. Buy from Supplier. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. Quant-iT PicoGreen dsDNA Assay Kit. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. • Suitable for use in 96- and 384-well plate formats. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. Rapidly detect as little as 0. Bioz Stars score: 99/100, based on 1 PubMed citations. 5 Q32851 15. 10 x 100 µL. Rapidly detect as little as 0. 3 Q33120 39. Rapidly detect as little as 0. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. • Suitable for use in 96- and 384-well plate formats. Bioz Stars score: 86/100, based on 1 PubMed citations. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Optimized for fluorescence microplate readers. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. Quant-iT PicoGreen dsDNA Assay Kit a. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). 99. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Rapidly detect as little as 0. Rapidly detect as little as 0. Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. Bioz Stars score: 86/100, based on 1 PubMed citations. A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. stranded DNA (dsDNA). • Suitable for use in 96- and 384-well plate formats. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. Catalog number: P7581. Description. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. 2 ng to 100 ng of DNA in the assay tube. For a standard. Picogreen Assay for DNA quantification: Which one is better to go for, a fresh. This was possible since, due to the low height of the light ray of only 8. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . Detecte rápidamente tan solo 0,25 pg/ml de ADNbc, incluso en presencia de ADNmc. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Assay Kit a. Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. This protocol is a simplified and c. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. dsDNA assays. Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays. ” application on Lunatic and compared to Qubit and. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5. It must be completely liquified before use by allowing it to come to room temperature. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. assay. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Common contaminants such as salts, free nucleotides, solvents,Product Description. お問い合わせ一覧 . 10 ng/mkl – 10000 ng/ml. Unit Size. 1 ng/μL to 2000 ng/μL, with an extended upper range to 4000 ng/μL. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. 522. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. Rapidly detect as little as 0. Price from $9. This protocol is a simplified and condensed. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. The Molecular Probes PicoGreen®. 86100 stars. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. 2. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Easy to use UV absorbance alternative assay that can be. 2. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. Effect of the buffer dilution on the DNA measurement with the Quant-It PicoGreen dsDNA assay. Description. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. 95 mL TE for the . quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. 2 – 100 ng. Thermo Fisher quant it picogreen dsdna quantitation assay kit. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The four reactions per sample were pooled, cleaned up with 1x AMPureXP beads, resuspended in 30 ul EB (10 mM Tris-HCl, pH 8. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Picogreen assays are compatible with most microplate readers and fluorometers. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. P7589) was used to quantify DNA by fluorescence. Barcoded amplicons were pooled in. • Suitable for use in 96- and 384-well plate formats. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. Plasma levels. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. Average 86 stars, based on 1. • Specific for dsDNA in the presence of RNA. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. 99 to $1999. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Table 2 summarizes results of the. with the Qubit dsDNA BR Assay Kit and 1 µL of each sample was measured in quadruplicate with the Quant-iT™ PicoGreen dsDNA Assay Kit. Rapidly detect as little as 0. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. • Suitable for use in 96- and 384-well plate formats. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 1 mL kit. 0 fluorometer according to the manufacturer’s protocols; a. 2022. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. For duplicate-well droplet. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). 2–100 ng, the fluorescence signal is linear with DNA. 2023. Description. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen dsDNA quantitation reagents are. Breadcrumb. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. P11495. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. Materials: 1. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. methods for the quantitation of DNA: the Invitrogen™ Quant-iT™ PicoGreen™ dsDNA assay and the Invitrogen™ Qubit™ dsDNA assays. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Using this data as a starting point, the optimal conditions (e. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. Mix by gently vortexing for 5 seconds. Product Type. 10pg/µl vs. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. high-range. 0, 20X c. 2 to 100 ng) Assay Kit were used with a Qubit 3. no. Double stranded DNA quantification performed in down to 2 µl using the LVis Plate. Briefly, 2 µL of sample was diluted in 498 µL TE. high-range. Picogreen assays are compatible with most microplate readers and fluorometers. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. no. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. 99 to $1999. Libraries were sequenced on the Illumina NovaSeq. dsDNA만을 선택적으로 정량. Quantifications using the UVette require half the amount ofCell viability was measured using a WST-8 assay (Cell Counting Kit-8, Dojindo). Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. 2 ng to 100 ng of DNA in the assay tube. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. High tolerance for contaminants. 2–100 ng, the fluorescence signal is linear with DNA. High linearity and sensitivity over a broad DNA range. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. ZERO BIAS . 5), 200X PicoGreen. 2–100 ng of DNA. Quantitation Range. 2. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. PicoGreen dsDNA quantitation reagents are. Qubit RNA XR Standard can be used with either Qubit RNA XR Assay.